ktrC [2017-04-24 15:34:12]

low affinity potassium transporter KtrC-KtrD, peripheric membrane component

locus: BSU14510

pI: 5.63

mw: 24.20 kDa

protein length: 221 aa

gene length: 663 bp

function: potassium uptake

product: low affinity potassium transporter KtrC-KtrD, peripheric membrane component (proton symport)

essential: no

synonyms: ylxV,yzaC,ykqB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,520,531 → 1,521,196

The protein

Paralogous protein(s)

• [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]

[SW|Domains]

• contains a [SW|RCK_N domain] at the N-terminus (according to [http://www.uniprot.org/uniprot/?query=RCK+N-terminal+domain&sort=score UniProt])
• contains a c-di-AMP-binding [SW|RCK_C domain] at the C-terminus (according to [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score UniProt]) [Pubmed|23671116]

Effectors of protein activity

• the protein binds c-di-AMP [Pubmed|23671116]

Structure

• [PDB|4J7C] (the [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB] complex, 56% identity) [Pubmed|23598340]
• [PDB|4XTT] (the ''S. aureus'' KtrA [SW|RCK_C domain] in complex with c-di-AMP, 57% identity) [Pubmed|25957408]

[SW|Localization]

• peripheral membrane protein [Pubmed|12562800]

Expression and Regulation

Operons

• genes: [gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]
  description: [Pubmed|12562800]

  regulation

  • constitutively expressed [Pubmed|12562800]

• genes: [gene|A656321846B2E0D1F39B528E2D8B8E620CCD1148|kinC]-[gene|22DBF4D1F93519A95E6BCC0FB1AC2387DF444E27|ykqA]-[gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8002614], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|8002615], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • constitutively expressed [Pubmed|12562800]

Biological materials

Mutant

• MGNA-A904 (ykqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/904 NBRP B. subtilis, Japan]
• GHB6 ([gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]::''spec''), [Pubmed|12562800] (available in [SW|Erhard Bremer]'s and [SW|Jörg Stülke]'s) labs, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A955&Search=1A955 BGSC] as 1A955
• GP2264 ([gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]::''ermC''), available in [SW|Jörg Stülke]'s lab
• GP2079 ([gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]::''tet''), available in [SW|Jörg Stülke]'s lab
• GP2083 (D([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB])::''aphA3'' D''[gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]''::''tet''), available in [SW|Jörg Stülke]'s lab

Expression vector

• pGP2907 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

Reviews

• 25869574,27935846

Original publications

• 12562800,23671116,23598340